Part:BBa_J37016

Predator Molecule Generator Sensing Test (GFP output)

Based around Imperial iGEM's Predator Cell in order to assay transfer function of pLux

[http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Test_Sensing_Predator_Construct Full Report on our project site]

Usage:

This part was created by iGEM@Imperial 2006 to be used as the predator sensing test construct in their 2006 project [http://openwetware.org/wiki/IGEM:IMPERIAL/2006 'Engineering a Molecular Predation Oscillator'] to characterize the sensitivity of the predator cell towards the prey moleculte Acyl-Homoserine Lactone (a quorum sensing molecule).

Biology:

In the presence of AHL, AHL and LuxR form a heterodimer, which activates the promoter luxpR and thus expression of the genes downstream of the promoter. Depending on the levels of AHL, the level of expression of LuxR and GFP vary.
The behaviour of this part was successfully modelled, generating a [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/project/Oscillator/project_browser/Test_Sensing_Predator_Construct/Modelling transfer curve].
The part was successfully tested using this [http://openwetware.org/wiki/IGEM:IMPERIAL/2006/Protocols/T9002 testing protocol] and [http://openwetware.org/wiki/IGEM:Imperial/Results/J37016 experimental data] from testing was documented.

Sequence and Features

Assembly Compatibility: